ChemoGenix assistance for your laboratory to conduct any screening in any cell type.
We primarily conduct proliferation-based screens using NALM6 cells; however, we recognize that
you may wish to explore other screening methods or different cell lines. We are in the process of
developing the capability to perform screens in additional cell lines, but this is not yet operational.
For the time being, we can assist your laboratory with experimental procedures and data analysis.
You will be responsible to acquire a Pooled Library of sgRNAs (AddGene has a large selection) and to
generate lentiviruses. We can only share reagents with you if you are employed at UdeM, so please
reach out to us if that applies. You can then infect and select any cell line relevant to your needs,
run the screen in appropriate conditions (proliferation-based, FACS cell sorting, any compound dosage,
timepoints, # of repeats…) and generate cell pellets. At every step, you can talk and consult with us.
At the end of the experiment you will then ship us the cell pellets and we will take care to extract
genomic DNA, PCR-amplify sgRNA sequences, do the NGS and compute the gene enrichment/depletion scores.
Academia & Industry: $1,200 CAD per pellet
Pooled library screenings are technically challenging experiments. They demand technical know-how and specialized expertise that not all labs have experience with. The two most challenging parts are (1) generating highly complex pools of high-titer lentivirus libraries to be used to infect enough cells to maintain pool representation at a low multiplicity of infection (MOI) and (2) sequencing sgRNAs sequences by NGS to monitor their frequencies and generate gene enrichment/depletion scores by bioinformatics. The first requires access to high efficiency electroporators, general knowledge about maintaining pool complexity, generation of high-quality lentiviruses and a method to properly assess MOI. The second requires access to Illumina sequencing devices and bioinformatics know-how to be able to analyze gigabytes of data. Although we are planning to provide high quality pooled infection-ready genome-wide KO lentivirus libraries of sgRNAs as well as controls to properly assess MOI, this service is not available yet. However, if you can already acquire any pooled sgRNA library and make lentivirus yourself for infecting any cell line of your choosing, ChemoGenix can help you with the 2nd part of screening.
Data Confidentiality
All clients retain exclusive and full ownership of their data.
Contact us
By nature, this service is very customizable. Please contact us
in order to discuss your needs and find the most appropriate solution.